Cytoplasmic transfer of RNA complementary to repetitious DNA sequences during early sea urchin embryogenesis
نویسندگان
چکیده
منابع مشابه
Alterations in chromatin structure during early sea urchin embryogenesis.
Sea urchin sperm before fertilization possess the longest nucleosome repeat length yet determined for any chromatin. By the time the fertilized egg gives rise to a blastula or gastrula embryo, the chromatin has a considerably shorter repeat length and, in addition, a sequence of different histone variants of H1, H2A, and H2B has appeared. We have investigated the relationship between these vari...
متن کاملThe expression of SpRunt during sea urchin embryogenesis
The runt box (runx) is a highly conserved DNA binding and protein-protein interaction domain that defines a family of heterodimeric transcription factors that regulate development in metazoans. The three mammalian runx genes are oncogenes with essential functions in normal development: Runx1 is required for hematopoiesis and is frequently mutated in human and murine leukemias; Runx2 is required...
متن کاملPiwi regulates Vasa accumulation during embryogenesis in the sea urchin.
BACKGROUND Piwi proteins are essential for germ line development, stem cell maintenance, and more recently found to function in epigenetic and somatic gene regulation. In the sea urchin Strongylocentrotus purpuratus, two Piwi proteins, Seawi and Piwi-like1, have been identified, yet their functional contributions have not been reported. RESULTS Here we found that Seawi protein was localized u...
متن کاملExogenous RNA is selectively retained in the small micromeres during sea urchin embryogenesis.
The small micromeres are multipotent cells of the sea urchin embryo that populate the coelomic pouches and contribute to various tissues of the adult (Juliano and Wessel, 2010). This lineage forms by two sequential unequal cleavages, divides only once prior to gastrulation, and accumulates several mRNAs and proteins selectively, including vasa, nanos, and piwi. In manipulating this embryo for e...
متن کاملNonrepetitive DNA sequence representation in sea urchin embryo messenger RNA.
Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusivel...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80862-2